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2 edition of Molecular determinants of synaptotagmin I clustering during synapse formation. found in the catalog.

Molecular determinants of synaptotagmin I clustering during synapse formation.

Peter Michael Gardzinski

Molecular determinants of synaptotagmin I clustering during synapse formation.

by Peter Michael Gardzinski

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Published .
Written in English


About the Edition

Little is known about the mechanisms leading to synaptotagmin I (syt) redistribution and synaptogenesis. Both C2 domains of syt bind with many effector molecules, although the C2B domain appears to play a more important role in neurotransmission than the C2A. Using an in vitro molluscan Lymnaea stagnalis synapse model system and immunohistochemistry, I show that syt localizes at the presynaptic contact site following membrane contact with an identified postsynaptic partner. Target-cell contact (TCC) induces an early transient upregulation in global expression of syt. Using a dominant negative approach, I show that loop 3 of the C2A prevents syt aggregation at the TCC site, whereas loop 3 of the C2B does not. A C-terminal peptide composed of the WHXL motif, important for vesicle docking, also prevents syt clustering. In conclusion, TCC is necessary for syt redistribution in nascent synapses between identified L. stagnalis neurons and requires loop 3 of the C2A.

The Physical Object
Pagination100 leaves.
Number of Pages100
ID Numbers
Open LibraryOL19217776M
ISBN 100494074914

  At the synapse, neurotransmitter release is mediated by the Ca 2+-induced fusion of transmitter-loaded synaptic vesicles with the presynaptic plasma plasma membrane–localized target (t)-SNAREs ([soluble N-ethylmaleimide–sensitive factor attachment protein 25 (SNAP) and syntaxin-1)] and the vesicle (v)–localized v-SNARE (synaptobrevin) and synaptotagmin Cited by: Synaptogenesis is the formation of synapses between neurons in the nervous system. Although it occurs throughout a healthy person's lifespan, an explosion of synapse formation occurs during early brain development, known as exuberant synaptogenesis. Synaptogenesis is particularly important during an individual's critical period, during which there is a certain degree of synaptic pruning due to .

Synaptotagmins Known as: Synaptotagmin, Synaptotagmins [Chemical/Ingredient], Tagmin A family of vesicular transport proteins characterized by an N-terminal transmembrane region and two C-terminal calcium-binding domains.   Regulated exocytosis forms the basis for many intercellular signaling processes, for example, in hormone secretion or neurotransmitter release. During regulated exocytosis, the membrane of a secretory vesicle fuses with the plasma membrane in a tightly controlled reaction that is most often triggered by calcium. Recent advances have allowed major insights into the molecular mechanisms Cited by:

Figure Structure of a synapse 4 Figure The schematic illustration of the synaptic vesicle recycling 7 Figure The structure of the C2 domains of synaptotagmin 1 13 Figure Stereo diagram of rat brain depicting the position of hippocampus 32 Figure Definition of EPSC charge and readily releasable pool (RRP) size   Synaptotagmin-7 is a candidate Ca2+ sensor for exocytosis that is at least partly localized to synapses. Similar to synaptotagmin-1, which functions as a Ca2+ sensor for fast synaptic vesicle (SV) exocytosis, synaptotagmin-7 contains C2A and C2B domains that exhibit Ca2+-dependent phospholipid binding. However, synaptotagmin-7 cannot replace synaptotagmin-1 as a Ca2+ Cited by:


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Molecular determinants of synaptotagmin I clustering during synapse formation by Peter Michael Gardzinski Download PDF EPUB FB2

Here we have investigated the role of the putative calcium sensor synaptotagmin I in vesicle aggregation during the formation of soma–soma synapses between identified partner cells using a simple in vitro synapse model in the mollusc Lymnaea stagnalis. Immunocytochemistry, optical imaging and electrophysiological recording techniques were used to monitor synapse formation and vesicle Cited by: Best characterized is synaptotagmin I, a resident of synaptic vesicles and neurosecretory granules.

Synaptotagmin I probably functions as an exocytotic Ca‐receptor that links the calcium signal to membrane fusion by means of Ca‐dependent binding to membranes and (possibly) to SNAREs.

Proper synapse formation in the central nervous system (CNS) during childhood provides the substrate for human perception, learning, memory, and cognition. Conversely, improper formation or function of these synapses leads to many disorders of learning and memory, including autism and other neurodevelopmental by: 2.

Although synapsins are abundant synaptic vesicle proteins that are widely used as markers of presynaptic terminals, the mechanisms that target synapsins to presynaptic terminals have not been elucidated. We have addressed this question by imaging the targeting of green fluorescent protein-tagged synapsins in cultured hippocampal neurons.

Whereas all synapsin isoforms targeted robustly Cited by: Synapse formation and plasticity depend on nuclear transcription and site-specific protein targeting, but the molecular mechanisms that coordinate these steps have not been well defined.

Neuron Article A Synaptotagmin Isoform Switch during the Development of an Identified CNS Synapse Olexiy Kochubey,1,* Norbert Babai,1,2 and Ralf Schneggenburger1,* 1Laboratory of Synaptic Mechanisms, Brain-Mind Institute, School of Life Sciences, E´cole Polytechnique Fe ´derale de Lausanne, Lausanne, Switzerland 2Institute of Animal Physiology & Department of Biology.

The synaptic vesicle (SV) protein synaptotagmin-1 (Syt1) is the Ca2+ sensor for fast synchronous by:   We show that synaptotagmin I, a protein resident in the vesicle membrane, remains clustered in isolated patches on the presynaptic membrane regardless of whether the Cited by: A further important realization from imaging studies has been that the assembly of synaptic structures occurs rapidly.

The time required from initial contact to establishment of a functional synapse is only in the range of 1–2 hours, with the formation of a presynaptic terminal occurring in only 10–20 minutes and the recruitment of postsynaptic neurotransmitter receptors taking place in.

Differential distribution of synaptotagmin immunoreactivity among synapses in the goldfish, salamander, and mouse retina RUTH HEIDELBERGER,1,* MENG M. WANG,2 and DAVID M. SHERRY2,* 1Department of.

Synaptotagmin-1 is localized to synaptic vesicles and is the trigger for their calcium-induced exocytosis. The two C2 domains of synaptotagmin-1 insert into the membrane upon calcium binding. How do we think synaptotagmin-1 induces membrane fusion.

5 molecular determinants and guided evolution of species‐specific rna editing (reenan, ) Most A‐to‐I RNA editing, which causes recoding, occurs in genes that affect neuronal signaling. Drosophila synaptotagmin I (dsytI) is the Ca 2+ sensor for neurotransmitter release (Yoshihara and Littleton, ) and a target for ADAR (Hoopengardner et al., ), with one exon containing four A‐to‐I RNA.

Synaptotagmin I function as a calcium regulator of release probability. Nature 41–49, doi: / Crossref PubMed ISI Google Scholar; Fierro J Jr, Haynes DR, Washbourne P. Ba is necessary for glutamatergic synapse formation in the sensorimotor circuit of developing by: 5.

Here we have investigated the role of the putative calcium sensor synaptotagmin I in vesicle aggregation during the formation of soma–soma synapses between identified partner cells using a simple in vitro synapse model in the mollusc Lymnaea stagnalis.

Immunocytochemistry, optical imaging and electrophysiological recording techniques were used to monitor synapse formation and Cited by: Monitoring Neurite Morphology and Synapse Formation in Primary Neurons for Neurotoxicity Assessments and Drug Screening Suk J.

Hong and Richik N. Ghosh Thermo Fisher Scientific • Pittsburgh, PA USA Application Notes C-AN_NT Abstract Synapse formation during nervous system development and degeneration.

Synaptotagmin is a synaptic vesicle-specific integral membrane protein that has been suggested to play a key role in synaptic vesicle docking and fusion. By monitor - ing Synaptotagmin’s cellular and subcellular distribu-tion during development, it is possible to study synaptic vesicle localization and transport, and synapse formation.

Calcium ion binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., ). [Supplied by OMIM] SYT1 is the master switch responsible for allowing the human brain to release s: SYT1, P65, SVP65, SYT, synaptotagmin.

The regulation of synaptic strength forms the basis of learning and memory, and is a key factor in understanding neuropathological processes that lead to cognitive decline and dementia.

While the mechanical aspects of neuronal development, particularly during axon growth and guidance, have been extensively studied, relatively little is known about the mechanical aspects of synapse formation Cited by: 8. Discordant to one-to-one homogenous synapse formation at the NMJ, the search, selection, and connectivity for specific synaptic partner in the CNS is more complex and intricate.

Thus, synapse formation in the CNS requires the collective actions of several molecules and the underlying cascade to ensure synapse specificity.

Expression and localization of CAST at the early stages of synapse formation. (A) Temporal expression of the CAZ proteins during synapse formation. The homogenates (20 μg of protein each) from rat brain tissues at embryonic day (E) 18, through postnatal day (P) 70 were analyzed by Western blotting using various Abs against the indicated by:.

Whereas little is known about the localization and function of stonin 1, recent work suggests that stonin 2 serves as a linker between the endocytic proteins AP‐2 and Eps15 and the calcium‐sensing synaptic vesicle (SV) protein synaptotagmin 1.

The molecular determinants involved in the recognition of SV cargo by the μ‐homology domain of Cited by: In this study, Li and colleagues demonstrate that synaptic vesicle fusion machinery, comprised of synaptotagmin-1, complexins, and synaptotagmin-7, in addition to determining the timing and Ca2+ dependence of neurotransmitter release, also dictates the properties of Cited by: Ca 2+-binding alters the protein-protein interactions of synaptotagmin such as increasing the affinity of synaptotagmin for syntaxin.

The C2B domain binds to phosphatidyl-inositol-3,4,5-triphosphate (PIP3) [ citation needed ] in the absence of calcium ions and to phosphatidylinositol bisphosphate (PIP2) in their presence, [13] suggesting that a lipid-interaction switch occurs during nome: